Laboratory diagnosis of malaria conventional and rapid diagnostic methods michael l. Laboratory diagnosis laboratory diagnosis of malaria is confirmed by the demonstration of. The role of the laboratory diagnosis of malaria is primarily to support clinical care. Feb 19, 2015 laboratory diagnosis of malarial parasite 1. Current available techniques can be separated in three categories. View and download powerpoint presentations on laboratory diagnosis of parasite ppt. The laboratory diagnosis of malaria captain eric denhoff medical corps, army of the united states in this clinic we wish to describe the methods which have been used with success in the laboratory diagnosis of malaria in a large army hospital operating in the south and southwest pacific theater of war. In malaria endemic areas, parasitemia does not ensure a diagnosis of severe malaria, because parasitemia can be incidental to other concurrent disease.
Laboratory diagnosis of malaria learn microbiology online. Renewed control efforts using a range of improved tools, such as longlasting insecticidetreated bednets and artemisininbased combination therapies, have more than halved the global burden of disease, but it remains high with 445 000 deaths and more than 200 million cases in 2016. Following unsuccessful eradication attempts there was a resurgence of malaria towards the end of the 20th century. Several different laboratory tests may be used to help diagnose malaria and monitor malaria treatment and for relapses. The two diagnostic approaches currently used most often, however, do not allow a satisfactory diagnosis of malaria. The epidemiology of malaria in china has changed dramatically 1,2, and the government embarked on a national malaria elimination programme in 2010 to eliminate malaria by 2020 in the country.
Rapid diagnostic tests rdt and microscopy are the primary diagnostic tools for the confirmation and management of suspected clinical malaria due to their high diagnostic performance in detection, their wide availability, and their relatively low cost. Laboratory diagnosis of malaria requires the identification of the parasite or its antigens products in the patients blood. The uploader spent hisher valuable time to create this malaria powerpoint presentation slides, to share hisher useful content with the world. Clinical diagnosis, the most widely used approach, is unreliable because the symptoms of malaria are very nonspeci. Prompt and accurate diagnosis is critical to the effective management of malaria and in improving the reliability of surveillance data. In malaria patients, a prompt and accurate diagnosis is the key to effective disease management. Parasitaemia counts will also be covered in the practicals along with the use of nonblood film methods of diagnosis, such as rapid diagnostic tests rdts and pcr. Multiplex realtime pcr for the diagnosis of malaria. This helps you give your presentation on malaria in a conference, a school lecture, a business proposal, in a webinar and business and professional representations the uploader spent hisher valuable time to create this malaria powerpoint. There are four main types of plasmodium p species that infect humans plasmodium vivax and plasmodium ovale, which cause a relapsing form of the disease, and. Recent investigations suggest that malarial retinopathy is better collective sensitivity of 95% and specificity of 90% than any other clinical or laboratory feature in distinguishing malarial. Malaria is the second most prevalent disease in pakistan resulting in 30,000 annual deaths. In endemic countries like pakistan precise and timely diagnosis of malaria is imperative to overcome.
These parasites are primarily spread by the bite of infected female anopheles mosquitos. Presidents malaria initiative pmiusaid ethiopia is implementing the malaria laboratory diagnosis and monitoring mldm project. Ppt malaria powerpoint presentation free to download id. The first step toward diagnosis and treatment of malaria is to consider malaria in the differential diagnosis. The uploader spent hisher valuable time to create this malaria parasite plasmodium powerpoint presentation slides, to share hisher useful content with the world. Salmonella is one of the most common causes of foodborne disease in the world. Basic malaria united states centers for disease control. Find powerpoint presentations and slides using the power of, find free presentations research about malaria diagnosis ppt.
In such areas malaria diagnosis is often made only on the basis of clinical symptoms although this is alarmingly inaccurate. The requirements of a diagnostic test are specificity, sensitivity, ease of performance and a reasonable cost. Field and laboratory comparative evaluation of rapid malaria. Microscopy including thick and thin blood films best method for diagnosing malaria. Field and laboratory comparative evaluation of rapid. The previous article described the clinical features and diagnosis of malaria. Laboratory diagnosis of malaria the diagnosis of malaria may in fact into two ways. Recent advances in the laboratory diagnosis of malaria btechan phd department of medical parasitology and entomology, faculty of medicine, national university of malaysia. Laboratory diagnosis of malaria 20 february 20 shridhan anand patil 2. Lab diagnosis of malaria parasites is the property of its rightful owner. Malarial parasite was screened in 128 malaria suspected patients and 150 healthy controls, by speciesspecific pcr, microscopy of blood smears, hemoanalyzer. Patients suspected of having malaria infection should be urgently evaluated.
Malaria presents a diagnostic challenge in most resource poor areas where malaria is endemic. One of the strategies was to establish a reliable malaria diagnosis laboratory network and a quality management system to support case verification and sourcetracking. Malaria is an infectious disease caused by plasmodium parasites. The adobe flash plugin is needed to view this content. The genus salmonella can be divided into two species s. However, there is a scarcity of qualified laboratory professionals for correctly diagnosing malaria using microscopy. For precise malaria diagnosis, several diagnostic approaches are employed in labs including microscopy, immuneflorescence technique, immunechromatographic testing ict, pcr, and use of hematological analyzers 710.
Establishing a china malaria diagnosis reference laboratory. Find powerpoint presentations and slides using the power of, find free. Laboratory diagnosis of malaria march 25, 2012 acharya tankeshwar lab diagnosis of parasitic disease, parasitology 0 once malaria is suspected on clinical grounds, it is mandatory to obtain the laboratory confirmation of the presence of malaria parasites in the patients specimen, whenever possible. The laboratory diagnosis of malaria captain eric denhoff medical corps, army of the united states in this clinic we wish to describe the methods which have been used with success in the laboratory diagnosis of malaria in a large army hospital operating in. Malaria is the second most prevalent disease in pakistan resulting in.
Performance evaluation of laboratory professionals on. Disease incidence depends on environmental suitability for local vectors in terms of altitude, climate, vegetation, and implementation of control measures, and hence is inextricably linked to. However, for a definitive diagnosis to be made, laboratory tests must demonstrate the malaria parasites or their components. W 25 iii preface 1 introduction 3 learning unit 1 malaria, the disease 7 learning unit 2 cleaning and storing microscope slides. Background microscopic diagnosis of giemsa stained thick and thin blood films by skilled microscopists has remained the gold standard laboratory method for the diagnosis of malaria. Laboratory diagnosis of malaria kashan university of. Therefore, malaria control programs, in addition to diagnosis and testing, must also target limiting spread of the disease through vector control. If so, share your ppt presentation slides online with. Previous article carcinogenesis with dibenzanthracene. It should be considered as a potential medical emergency and should be treated accordingly, delay in diagnosis and treatment is a leading cause of death in malaria patient. Development of guidelines and standardized training materials. It is transmitted by the bite of the female anopheles mosquito. Practicals will be complimented with relevant lectures on malaria as a disease, laboratory diagnosis and the role of serology and molecular diagnosis. Malaria is a disease of tropical and subtropical regions, having been eradicated from temperate countries steadily over the last 100 years.
Powerpoint is the worlds most popular presentation software which can let you create professional malaria powerpoint presentation easily and in no time. Morphological comparison and images of plasmodium species. Free download malaria parasite plasmodium powerpoint. The genus salmonella is a member of the family enterobacteriaceae. A free powerpoint ppt presentation displayed as a flash slide show on id. Prevention and treatment of malaria is more complex due to the emergence of drug resistance, pesticide resistant mosquito vectors, and large populations of infected people in many areas of the world. Although malaria control efforts have been successful in some regions, malaria remains one of the most important causes of death in subsaharan africa, particularly in women and children. Learn about malaria, an infection of the blood caused by plasmodium parasites. The role of the biomedical scientist in the diagnosis of malaria. Treatment for malaria should not be initiated until the diagnosis has been confirmed by laboratory investigations. The continued success of the programme will depend upon the accurate diagnosis of the disease in the laboratory. The world health organization estimates that two billion people are at. Malaria can be suspected based on the patients symptoms and physical findings at. The global control of malaria is more challenging than that of many other infectious diseases.
However, for a definite diagnosis, the malaria parasite must be seen in a blood film. For routine malaria microscopy a thin and a thick film are made on the same slide. Delay in diagnosis and treatment is a leading cause of death in malaria patients in the united states. In this article we cover laboratory tests used to diagnose malaria 1, 2. Basic malaria united states centers for disease control and. Laboratory diagnosis of malaria plasmodium falciparum plasmodium vivax plasmodium malariae. In china, the prevalence of malaria has reduced dramatically due to the elimination programme. Introduction although serological methods are well.
Performance of microscopy for the diagnosis of malaria and. This practical guidance for clinical microbiology document on the laboratory diagnosis of parasites from the gastrointestinal tract provides practical information for the recovery and identification of relevant human parasites. In endemic countries like pakistan precise and timely diagnosis of malaria is imperative to overcome the associated risks of fatal outcomes. View and download powerpoint presentations on malaria diagnosis ppt.
Malaria is a disease of global importance that results in 300 660 million cases annually and an estimated 2. Sep 16, 2011 the role of the biomedical scientist in the diagnosis of malaria. Malaria symptoms symptoms of malaria may include fever, chills, vomiting, diarrhoea, cough, stomach, pain and muscular aches and weakness. Malaria is generally diagnosed by microscopy and rapid antigen testing. Salmonella is a gramnegative, rodshaped, motile bacilli which move with the use of its peritrichous flagella. Malaria must be recognized promptly in order to treat the patient in time and prevent further spreading of infection in the community. Half of th e worlds population is at risk from malaria accounting for 2 50 million cases per year causing 8,60,000 deaths. The basic requirements for this are a reliable malaria diagnosis laboratory network and quality management system to support case verification and source tracking. In pakistan laboratory diagnosis of malaria is indispensable to avoid misdiagnosis as per national guidelines. Microscopic identification is the method most frequently used to demonstrate an active infection. Powerpoint is the worlds most popular presentation software which can let you create professional malaria parasite plasmodium powerpoint presentation easily and in no time. Laboratory diagnosis of mycobacterium tuberculosis. Technical aspects of laboratory tools for diagnosis of malaria are discussed further separately.
Malaria, diagnosis, serology, monoclonal antibodies, dna probes. Apr 16, 2016 malaria can be diagnosed by demonstration of the parasitering form,schizonts,gametocytes etc from the peripheral blood film and detection of antigen by. Find powerpoint presentations and slides using the power of, find free presentations research about laboratory diagnosis of parasite ppt. Plasmodium malariae and plasmodium falciparum, which do not cause relapses. The epidemiology, pathogenesis, diagnosis, and treatment of malaria are discussed separately. This helps you give your presentation on malaria parasite plasmodium in a conference, a school lecture, a business proposal, in a webinar and business and professional representations. View and free download mahabharata powerpoint presentation slides. Rapid diagnosis and prompt effective treatment are essential patients who reside in or have traveled to southeast asia receive intensive management as appropriate for severe falciparum malaria clin infect dis. Diagnosis of tuberculosis in children is challenging. A comparative study of thick and thin blood films in the diagnosis of scanty malaria parasitaemia. If infected with the malaria parasite, plasmodium results in the most severe form of malaria and if left untreated, it can cause serous illnesses. The aim of this study was to evaluate the performance of laboratory professionals on malaria.
Free download malaria powerpoint presentation slides. Malaria can be suspected based on the patients travel history, symptoms, and the physical findings at examination. In the present study, the contribution of a quantitative multiplex malaria pcr was investigated. Ppt malaria powerpoint presentation free to download. The implementation of malaria laboratory diagnosis and monitoring activities was key to improving the quality of malaria laboratory diagnosis and patient management in the country in general and supported health care facilities in particular. Clinical diagnosis lacks standardization, and traditional and molecular microbiologic methods lack sensitivity, particularly in children. Laboratory diagnosis of malarial parasite linkedin slideshare. The diagnosis of malaria should also be considered in any person with fever of unknown origin regardless of travel history. Clinical diagnosis microscopic diagnosis blood smear fluorescent microscopy quantitative buffy coat qbc 2021915 vaidegi. Who recommends parasitological diagnosis of malaria. Introduction light microscopy antigen detection rapid malaria diagnosis tests rdts serology other qbc pcr summary 3.
1068 493 452 711 974 465 166 55 1276 528 862 935 569 1345 282 1115 1546 410 806 1475 991 675 980 556 1216 526 584 912 1095